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| Prepared by enzymatic synthesis from N-acetylneuraminic acid and lactose.*1
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C23H39NO19 (MW : 633.55)
![N-Acetylneuraminyl-α (2→6)-lactose [6'-Sialyllactose]](image/na_kigo01.gif "N-Acetylneuraminyl-α (2→6)-lactose [6'-Sialyllactose]")
NeuAc α (2→6)Gal β (2→6)Glc |
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| White amorphous powder. |
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More than 98% by HPLC
Homogeneous by thin layer chromatographic analysis. |
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1. Thin layer chromatography (TLC)
| TLC plate |
Silica Gel K5 (Whatman) |
| Solvent system |
n-Propanol : 1 M NH4OH : H2O (6 : 2 : 1) |
| Color reagent |
Resorcinol-hydrochloric acid*2 |
2. High performance liquid chromatography (HPLC)
| Column |
Cosmosil 5NH2 (4.6 mm ID x 150 mm) |
| Eluent |
Acetonitrile 20 mM KH2PO4 (70 : 30) |
| Detection |
210 nm |
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| Stable for one year when stored on desiccated condition below 5 ºC. |
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| *1. |
I. Maru, Y. Ohta, K. Okamoto, S. Suzuki, K. Kakehi, and Y. Tsukada, Biosci. Biotech. Biochem., 56, 1557 (1992) |
| *2. |
L. Svennerholm, Biochim. Biophys. Acta, 24, 604 (1957) |
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| 1 mg |
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![N-Acetylneuraminyl-α (2→6)-lactose [6'-Sialyllactose]](image/na_title.gif "N-Acetylneuraminyl-α (2→6)-lactose [6'-Sialyllactose]"){kind=small}
