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![N-Acetylneuraminic acid [NANA;Sialic acid;NeuAc]](image/nj_title.gif "N-Acetylneuraminic acid [NANA;Sialic acid;NeuAc]")
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Prepared by enzymatic hydrolysis of colominic acid*1, or enzymatic synthesis from N-acetylglucosamine and pyruvic acid.*2
Purified by ion-exchange column chromatography and crystallization.
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| C11H19NO9 (MW : 309.27)
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| White crystalline powder. |
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| More than 99% by colorimetric determination and HPLC. |
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1. Colorimetric assay
TBA method*3-*4 is generally used to measure free sialic acid.
Resorcinol method*5can be used to measure free, bound, or total sialic acid.
2. High performance liquid chromatography (HPLC)
| Column |
Bio-Rad Aminex HPX-87H |
| Eluent |
10 mM H2SO4 |
| Detection |
205 nm |
3. Enzymatic assay
NANA aldolase method*6
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| Authentic specimen of the highest purity, substrate for NANA aldolase and starting material for preparing NANA derivatives. |
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| Stable for one year when stored on desiccated condition at 5 ºC. |
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| *1. |
Y. Uchida, Y. T sukada, and T. Sugimori, Biochim. Biophys. Acta., 350, 425 (1974) |
| *2. |
I. Maru, J. Ohnishi, Y. Ohta and Y. Tsukada, Carbohydr. Res., 306, 575 (1998) |
| *3. |
Y. Uchida, Y. Tsukada and T. Sugimori, J. Biochem., 82, 1425 (1977) |
| *4. |
L. Warren, J. Biol. Chem., 234, 1971 (1959) |
| *5. |
J. T. Cassidy, G. W. Jourdian and S. Roseman, J. Biol. Chem., 240, 3501 (1965) |
| *6. |
H. Araki and M. Yamada, Methods of Enzymatic Analysis, VI, 80 (1984) |
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From colominic acid: 100 mg, 1 g, Bulk
Enzymation: 1 g, 10 g, 100 g, 1 kg, Bulk |
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![N-Acetylneuraminic acid [NANA;Sialic acid;NeuAc]](image/nj_kigo01.gif "N-Acetylneuraminic acid [NANA;Sialic acid;NeuAc]")
